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通過(guò)谷歌學(xué)術(shù)搜索發(fā)現(xiàn)，自2010年以來(lái)，就有大量科研人員使用Simgen產(chǎn)品進(jìn)行科學(xué)研究并發(fā)表學(xué)術(shù)論文。不僅研究論文數(shù)量巨大，而且有一批高質(zhì)量的注明使用Simgen產(chǎn)品的研究論文發(fā)表在Cell、Nature、Cell Stem Cell、Immunity、Developmental Cell、Cell Reports、Nature Medicine、Nature Immunology、Nature Chemical Biology、Nature Cell Biology、Nature Communications、PNAS、Blood、Gastroenterology、Nature Protocols、Plant Cell、Hepatology等高水平期刊上。
所有Simgen員工為此感到歡欣和鼓舞，感到驕傲和自豪！廣大用戶(hù)大量標(biāo)注Simgen的研究論文的發(fā)表是對(duì)所有Simgen最大的支持和鼓勵(lì)，最真切的認(rèn)可和回報(bào)！
作為Simgen人，我們會(huì)再接再厲，不斷開(kāi)拓進(jìn)取，為廣大用戶(hù)提供更多高質(zhì)量的產(chǎn)品和服務(wù)，繼續(xù)踏踏實(shí)實(shí)地為中國(guó)的生命科學(xué)事業(yè)添磚加瓦，以答謝廣大用戶(hù)對(duì)我們的支持和鼓勵(lì)，對(duì)我們的認(rèn)可和回報(bào)!
Simgen將永遠(yuǎn)和您一起為中國(guó)的生命科學(xué)事業(yè)的騰飛貢獻(xiàn)自己的一份力量?。?！
LJ Deng, WX Ouyang, R Liu, M Deng, JW Qiu… - Liver …, 2021 - Wiley Online Library
… Peripheral blood of 2 mL was collected from each subject, and the genomic DNA was
extracted using a DNA extraction kit (Simgen, China) according to the manufacturer’s instructions. …
YH Zheng, XB Cai, LQ Xia, FY Zhou, XR Wen… - Molecular …, 2021 - ncbi.nlm.nih.gov
… in peripheral blood by using the Blood DNA Mini Kit (Simgen, Hangzhou, China) [30]. …
The genomic DNA was sheared into 100 base pairs via the Exome Enrichment V5 Kit (Agilent …
J Bai, Q Ma, Y Lan, Y Chen, S Ma, J Li, C Liu… - Frontiers in Cell and …, 2021 - frontiersin.org
… From each subject, 400 μL whole blood was treated according to the SimGEN Blood DNA
… The human ELISA kit was produced by Abcam. A standard sample or plasma, horseradish …
B Chen, X Yu, X Zhang, H Yang, Y Cui… - Journal of …, 2022 - hindawi.com
… ese tubes were preserved at ?80C before genomic DNA was extracted with a Simgen DNA
Blood Mini Kit (Simgen, Hangzhou, China) according to the manufacturer’s instructions. All …
Y Xiang, B Liang, X Zhang, X Qiu… - Clinical …, 2022 - clinicalepigeneticsjournal …
… Genomic DNA was extracted using the phenol/chloroform method or a commercial DNA
extraction kit (3001050, Simgen, Hangzhou, China) based on the sample size and was …
6. Novel SOX2 mutation in autosomal dominant cataract-microcornea syndrome
ZB Lin, J Li, L Ye, HS Sun, A Yu, SH Chen, FF Li - BMC ophthalmology, 2022 - Springer
… A conventional DNA extraction kit (Simgen, Hangzhou, China) was used for genomic
DNA extraction. In addition, blood samples from another 200 healthy controls without of eye …
1.The complete mitochondrial genome of the common strain black carp (Cyprinus carpio?var. baisenensis)
LJ Deng, WX Ouyang, R Liu, M Deng, JW Qiu… - Liver …, 2021 - Wiley Online Library
… Peripheral blood of 2 mL was collected from each subject, and the genomic DNA was
extracted using a DNA extraction kit (Simgen, China) according to the manufacturer’s instructions. …
T Xiong, Y Zeng, W Wang, P Li, Y Gai, C Jiao, Z Zhu… - Journal of Fungi, 2021 - mdpi.com
… The scraped mycelia were lyophilized, and then the DNA was extracted using a SIMGEN
Fungi Kit (Simgen, Hangzhou, China) according to the manufacturer’s protocol. The extracted
Y Zeng, T Xiong, B Liu, E Carstens, X Chen… - …, 2021 - Am Phytopath Society
… The mycelia were scraped from the plates and lyophilized before DNA extraction with a
SIMGEN Fungi Kit (Simgen, Hangzhou, China) according to the manufacturer’s protocol. The …
W Zhu, M Yu, R Xu, K Bi, S Yu, C Xiong… - Environmental …, 2022 - Wiley Online Library
… Total RNA of fungi and plant samples were isolated using RN03-RNApure Kit (Aidlab, …
cinerea strains was isolated using Fungal Genomic DNA Purification Kit (Simgen, Hangzhou…
H Weng, Y Liu, I Captoline, X Li, D Ye, R Wu - Computers and Electronics in …, 2021 - Elsevier
… it. The DNA was extracted using a Plant DNA kit (Simgen, Hangzhou, China). TaqMan
probes and primer pairs were synthesized according to the reported study by a
biotechnology company (Tsingke, Hangzhou, China). The …
W Yang, J Ren, W Liu, D Liu, K Xie, F Zhang… - Horticultural Plant …, 2022 - Elsevier
… The genomic DNA was isolated from citrus protoplasts and the mutant shoots using plant
DNA Kit (SIMGEN, China). The genomic fragments containing the target sites were amplified by …
3.Microbial diversity on the surface of historical monuments in Lingyan Temple, Jinan, China
T Li, Y Cai, Q Ma - Microbial Ecology, 2022 - Springer
… Total genomic DNA was extracted with a Plant DNA Isolation Kit (Simgen, Hangzhou, China)
according to the manufacturer’s protocols. The DNA was subsequently used as a template …
4.The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements.
W Ren, Z Jiang, M Zhang, L Kong, H Zhang… - Frontiers in plant …, 2022 - europepmc.org
… Total genomic DNA was extracted using the Plant DNA Extraction Kit (SIMGEN, Hangzhou,
China) according to the manufacturer’s instructions. DNA purity and integrity were verified via …
Y Cai, H Cao, F Wang, Y Zhang, P Kapranov - Nature communications, 2022 - nature.com
… Genomic DNA from sperm cells was extracted using a Sperm DNA Purification Kit (Simgen,
4202050) following the manufacturer’s instructions. All DNA samples were eluted in …
J Wu, Y Yin, M Qin, K Li, F Liu, X Zhou… - Shock (Augusta …, 2021 - ncbi.nlm.nih.gov
… gut tissues using TRIzol reagent (Simgen, China). The same amount of total RNA was
reverse transcribed to cDNA using the ReverTra Ace qPCR RT kit (Simgen, China). Quantitative …
L Liang, Q Xiong, J Kong, C Tian, L Miao… - Biomedicine & …, 2021 - Elsevier
… isolated using TRIzol reagent (Simgen, Hangzhou, China) … using a Whole Protein
Extraction kit (KeyGen BioTech, China) … an enhanced chemiluminescence detection kit (biosharp, …
Z Yu, YY Zhao, N Jiang, AZ Zhang, MY Li - Fish & Shellfish Immunology, 2021 - Elsevier
… kits (Shanghai Biotechnology Co., Ltd., Shanghai, China). All measurement steps were
performed according to the relevant kit … extracted using TRIzol Reagent (simgen) according to the …、
4.A transcription factor DAF-5 functions in Haemonchus contortus development
W Di, F Li, L He, C Wang, C Zhou… - Parasites & …, 2021 - parasitesandvectors.biomedcentral …
… contortus using TRIzol reagent (Simgen, China). All RNA samples were treated with
DNase I to remove genomic DNA (gDNA), and then their integrity and yields were verified by …
Y Jiang, Z Peng, Q Man, S Wang… - Biology of sex …, 2022 - bsd.biomedcentral.com
… Total RNA was extracted using TRIzol reagent (Simgen) and cDNA was prepared using
PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). Analysis of mRNA expression …
6.中國(guó)南方一罕見(jiàn)遲發(fā)性脊椎骨骺發(fā)育不良大家系的表型和基因型的相關(guān)性研究
黃穎， 謝杰， 鄭詩(shī)瑤， 唐佳， 郭奕斌 - 中山大學(xué)學(xué)報(bào)(醫(yī)學(xué)科學(xué)版), 2022 - researchgate.net
...1.2.3 逆轉(zhuǎn)錄 PCR（RT-PCR）使 用 Trizol 試 劑（Simgen 試劑盒），從受試者新鮮外周血白細(xì)胞中提
取總 RNA，在 20 μL 體積內(nèi)反向轉(zhuǎn)錄 1 000 ng RNA（Fermantas 試劑盒）...
A Ayaz, H Huang, M Zheng, W Zaman, D Li… - International journal of …, 2021 - mdpi.com
… Triplicate biological samples of either soybean or Arabidopsis were used for the extraction
of total RNA using the Trizol total RNA extraction kit (SIMGEN, Beijing, China) according to …
2.A novel homozygous mutation in the PADI6 gene causes early embryo arrest
X Wang, H Zhu, Y He, J Zeng, J Zhao, Q Xia, L Wu… - Reproductive health, 2022 - Springer
… after transfection using the Total RNA Purification Kit (SimGen). Reverse transcription and
amplification were performed using the Evo M-MLV RT Mix Kit (Accurate Biotechnology), and …
Y Tian, B Wu, L Peng, J Wang, M Shen - Frontiers in Immunology, 2022 - ncbi.nlm.nih.gov
… Total RNA was extracted from frozen blood using an RNA isolation kit (Simgen, 5201005).
RNA was reverse transcribed using a 1st Strand cDNA Synthesis Kit (Takara, 6110). The …
2.Role of NINJ1 in Gout Flare and Potential as a Drug Target
H Zhang, J Gao, W Fang, Y Tang, X Fang… - Journal of …, 2022 - dovepress.com
… Total RNA was isolated from whole blood using a whole Blood RNA isolation kit (Simgen),
total RNA was isolated from cultured cells using Hipure total RNA mini kit (Magen). Isolated …
Y Yin, X Liu, Q Tian, Y Fan, Z Ye, T Meng, G Wei…- medRxiv, 2022 - medrxiv.org
… Total RNA was isolated from 2.5ml blood using total RNA isolation kit from SIMGEN. Isolated total RNA and remaining blood sample were subjected to mRNA sequencing and whole-…
Q Zhang,S Li, P Lei, Z Li, F Chen, Q Chen, Y Wang… - Virologica Sinica, 2021 - Springer
… Three micrograms of RNA was reverse transcribed into cDNA using Toyobo RTkits with a …in each supernatant, viral nucleic acid isolation kits (Simgen, China) were used to extract the …
X Liu, J Liang, Y Yu,X Han, L Yu, F Chen… - Journal of Medicinal …, 2022 - ACS Publications… Supernatant virion RNA was extracted with the Viral Nucleic Acid Purification Kit (Simgen) according to the manufacturer’s instructions. Viral RNA was reverse transcribed into cDNA by …
J Feng, D He, T Chen, W Cai, Y Chen, Y Xiong, Q Wu… - 2021 - researchsquare.com
… The contents in the colon of the piglets was aseptically collected, and the total DNA of the colonic contents was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the …
R Zhen, C Liu, C Wei, Y Luo, X Hu, G Liu…- Journal of Applied …, 2022 - Wiley Online Library
… The feces of the piglets were aseptically collected, and the total DNA of the feces 111 was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the 112 instructions …
3.Effects of Niacin on Resistance to Enterotoxigenic Escherichia coli Infection in Weaned Piglets
R Zhen, J Feng, D He, Y Chen, T Chen, W Cai… - Frontiers in …, 2022 - ncbi.nlm.nih.gov
… The contents in the colon of the piglets were aseptically collected, and the total DNA of the
colonic contents was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the …
1.Genome-wide characterization of C2H2 zinc-finger gene family provides insight into the mechanisms and evolution of the dehydration–rehydration responses in Physcomitrium and Arabidopsis
Xuan Li, Cao, Jialing Li, Qinqin Niu, Yuanping Mo and L Xiao - researchgate.net
cDNA was generated using HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme, China). The gene… study were extracted and purified usin the Simgen Plasmid Extract Kit (Simgen, China). …
Y Yao, S Deng, F Zhu- Genes, 2022 - mdpi.com
… Subsequently, products were excised from the gel, purified with a commercial kit (DNA Gel ExtractionKit, 2001250, SIMGEN, Hangzhou, China) and sequenced by Sanger sequencing. …
2.Clinical Performance of Nanopore Targeted Sequencing for Diagnosing Infectious Diseases
Y Fu, Q Chen, M Xiong, J Zhao, S Shen…- Microbiology …, 2022 - Am Soc MicrobiolL Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com
… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mix kit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, …
2.Polymeric immunoglobulin receptor in dongtingking crucian carp (Carassius auratus indigentiaus): Molecular characterization and expression analysis in response to?
H Xia, P Yang, Z Chen, Y Zhang, L Liu… - Aquaculture …, 2022 - Wiley Online Library …
...The total RNA was extracted using the animal tissue total RNA extraction kit (Simgen). RNA … Reverse transcription was performed usinga reverse transcription kit (Simgen)and the …
1.Integrated transcriptome sequencing and RNA interference reveals molecular changes in?Diaphorina citri?after exposure to validamycin
HZ Yu, YX Xie, J Wang, Y Wang, YM Du… - Insect …, 2021 - Wiley Online Library… Total RNA was isolated from the control group (CK), V12, V24, and V36 using an animal tissue total RNA kit (Simgen, Hangzhou, China) following the manufacturer's protocol. In this …
2.Silencing of?Glycogen Synthase Kinase 3?Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid,?Diaphorina citri
JB Zhang, ZJ Lu, HZ Yu- International journal of molecular sciences, 2022 - mdpi.com
Thetotal RNA was isolated from D. citri in different tissues (midgut, leg, head, wing,integumentand fat body) and at different developmental stages (egg, 1st-, 2nd-, 3rd-,4th-and 5th-instar nymphs and adults) using the animal tissue total RNA kit (Simgen).
3.Polymeric immunoglobulin receptor in dongtingking crucian carp (Carassius auratus indigentiaus): Molecular characterization and expression analysis in response to?
H Xia, P Yang, Z Chen, Y Zhang, L Liu… - Aquaculture …, 2022 - Wiley Online Library
… The total RNA was extracted using the animal tissue total RNA extraction kit (Simgen). RNA… Reverse transcription was performed using a reverse transcription kit (Simgen)and the …
P Wang, X Jia, X Xiao, S Li, Y Long, M Liu…- Frontiers in Cell and …, 2021 - frontiersin.org… (HeLa) according to the manufacturer’s instruction (Rapid Plasmid Mini Kit, SIMGEN). The transfected cells were cultured for 48 h and then collected for transcript expression analysis. …
2.The identification of a novel splicing mutation in the DMD gene of a Chinese family
W Liu, X Shi, Y Li, F Qiao, Y Wu- Clinical Case Reports, 2021 - Wiley Online Library
… The positive colonies were used for extracting plasmid by Rapid Mini Plasmid Kit (Simgen),and plasmid was subsequently sequenced to confirm the inserts. The confirmed plasmids …
3.A Novel Mutation c. 3392G> T of COL2A1 Causes Spondyloepiphyseal Dysplasia Congenital by Affecting Pre-mRNA Splicing
L Fan, L Ji, Y Xu, G Shen, K Tang, Z Li, S Ye…- Frontiers in …, 2022 - ncbi.nlm.nih.gov… The wild-type and mutated amplified products were purified by a gel extractionkit and then … After plasmid amplification in DH5α competent cells and plasmid extraction (SIMGEN), the …
L Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mixkit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, …
2.Conjugate of ibrutinib with a TLR7 agonist suppresses melanoma progression and enhances antitumor immunity
S Ren, X Wang, G Jin- International journal of biological sciences, 2022 - ncbi.nlm.nih.gov
… cDNA synthesis and real-time PCR were performed using a cDNA synthesis master mixkit and 2×SYBR Green PCR Mix (Simgen, Hangzhou, China). The following primers were used: c…
3.甜橙SPL 基因家族的鑒定及其在成花誘導(dǎo)中的表達(dá)分析
楊杰， 陳蓉， 胡文娟， 吳巧玲， 陳健美…- 西北植物學(xué)報(bào), 2022 -
… 使用植物總RNA 提取試劑盒(Simgen,中國(guó)杭州) 提取樣品總RNA,使用反轉(zhuǎn)錄試劑盒(Simgen,中國(guó)杭州)反轉(zhuǎn)錄合成cDNA.利用2×SYBR Green PCR Mix(Simgen,中國(guó)杭州)在Light-Cycler480 …
1.Crosstalk between the gut microbiome and clinical response in locally advanced thoracic esophageal squamous cell carcinoma during neoadjuvant?…
L Xu, Y Qi, Y Jiang, Y Ji, Q Zhao, J Wu…- Annals of …, 2022 - ncbi.nlm.nih.gov
… The samples were dissolved in into fecal DNA preservation fluid (Simgen, 4103100,Hangzhou, China), transported to the laboratory, and stored at ?8 ℃ for subsequent processing. …
1.Trigeminal Nerve Derived-Substance P Regulates Biological Characteristics of Limbal Stem Cells by Modulating the PI3K-AKT Signaling Pathway
P Xu, X Lin, X Dong, Y Liu, Z Wang, S Wang - Available at SSRN 4237970 - papers.ssrn.com
… Limbal tissues were quickly dissected and placed in RNA later reagent (Simgen, Hangzhou,China)for RNA isolation. Each sample contained three to five independent repeated mice …
·常溫保存1周，37℃保存1天，4℃至少保存1個(gè)月，組織4℃浸泡過(guò)夜后-20℃或-80℃可長(zhǎng)期保存?！し磸?fù)凍融：凍存于-20℃或-80℃的組織可反復(fù)凍融20次而不影響RNA提取的質(zhì)量。 查看產(chǎn)品
·15分鐘內(nèi)即可完成血液總DNA的制備·無(wú)須事先分離去除紅細(xì)胞及蛋白酶K消化步驟，可室溫運(yùn)輸儲(chǔ)存·徹底清除血樣中的PCR抑制物，可使用多至1/2反應(yīng)體系體積的模板進(jìn)行擴(kuò)增 查看產(chǎn)品
·樣本通用性強(qiáng)：植物、真菌、糞便、細(xì)菌、動(dòng)物組織、血液均可適用·樣本取樣范圍大：50~200 mg均可適用，特別適合從無(wú)法徹底溶解的生物樣本中提取總DNA·操作步驟簡(jiǎn)潔、快速，無(wú)需蛋白酶K消化及添加乙醇步驟，30-40分鐘內(nèi)即可完成植物總DNA的制備·特殊設(shè)計(jì)的溶解液有效應(yīng)對(duì)植物中的多糖衍生物的干擾·特別優(yōu)化配置的核酸純化柱僅高效吸附大片 查看產(chǎn)品
物組織DNA試劑盒-ut-20250120034241.jpg)
·經(jīng)典蛋白酶K消化步驟，適合從各種不同來(lái)源的動(dòng)物組織（包括鼠尾、鼠耳、福爾馬林固定組織等）中分離純化DNA·可從體液樣本（包括抗凝全血、唾液、培養(yǎng)細(xì)胞懸液等）分離純化DNA·可從昆蟲(chóng)、革蘭氏陰性菌或革蘭氏陽(yáng)性菌中分離純化DNA· 無(wú)須酚氯仿抽提及異丙醇沉淀步驟 查看產(chǎn)品
· 20-40分鐘內(nèi)即可完成體液中總核酸的制備，同步分離純化病毒DNA或病毒RNA· 較傳統(tǒng)的煮沸法提取病毒DNA相比，檢測(cè)靈敏度增加10-50倍以上. 較傳統(tǒng)Trizol法提取病毒RNA相比，檢測(cè)靈敏度增加5-10倍以上· 添加的Carrier RNA有效提高病毒的檢測(cè)敏感性（穩(wěn)定檢測(cè)到體液中濃度為100 copies/ml的病毒）· 無(wú)須酚氯仿抽提及異丙醇沉淀步驟· 所獲的核酸不含雜質(zhì)及抑制物 查看產(chǎn)品
·40-50分鐘內(nèi)即可完成糞便總DNA的分離與純化·可同步分離純化人或動(dòng)物腸道脫落細(xì)胞的DNA、細(xì)菌DNA、病毒DNA及寄生蟲(chóng)DNA·無(wú)須酚氯仿抽提及異丙醇沉淀步驟·柱純化技術(shù)徹底去除糞便中PCR抑制物 查看產(chǎn)品
· 20-40分鐘內(nèi)即可完成精液總DNA的制備?！?可從新鮮或者是冷凍的精液中分離純化總DNA?！?直接從200 μl精液樣本中分離純化DNA，無(wú)需事先分離精子細(xì)胞?！?核酸純化住可最大吸附20 μg總DNA?！?所獲得DNA長(zhǎng)度在200bp-50kb之間，主要的長(zhǎng)度在30kb左右。 查看產(chǎn)品
·添加的Carrier RNA能有效提高DNA的回收效率·適用于各種不同來(lái)源的含微量DNA的樣本·無(wú)須酚氯仿抽提及異丙醇沉淀步驟·25 μl微量洗脫體積洗脫核酸·可用于指紋印跡和親子鑒定等基因分型實(shí)驗(yàn) 查看產(chǎn)品
·20-40分鐘內(nèi)即可完成全血中總RNA的分離與純化·無(wú)須事先裂解去除紅細(xì)胞·全血樣品可用裂解液裂解后﹣20℃保存一周后再使用·所獲的RNA不含雜質(zhì)及抑制物，模板可多加至反應(yīng)體系的1/2體積進(jìn)行擴(kuò)增 查看產(chǎn)品
·20-40分鐘內(nèi)即可完成總RNA的分離與純化·經(jīng)典酸性酚萃取法與柱純化法的完美結(jié)合·無(wú)需氯仿，配置的Buffer EX可完美替代氯仿·樣本：動(dòng)物組織、細(xì)胞、植物組織、血液等·應(yīng)用：RT-PCR、Northern Blot、芯片分析等 查看產(chǎn)品
·經(jīng)典總RNA分離純化方法·樣本：血液、動(dòng)物細(xì)胞、組織、植物組織等·適合從各種不同數(shù)量級(jí)的實(shí)驗(yàn)樣本中分離純化總RNA應(yīng)用：RT-PCR、Northern Blot、芯片分析 查看產(chǎn)品
·1小時(shí)內(nèi)即可完成miRNA的分離與純化·柱分離技術(shù)快速高效、準(zhǔn)確分離20-200nt的miRNA 片段·柱純化技術(shù)確保獲得高純度的miRNA·應(yīng)用：RT-PCR、Real-time RT-PCR、Northern Blot、 芯片分析 查看產(chǎn)品
·提供供兩套裂解液體系，可以解決各種簡(jiǎn)單的植物組織、富含多糖多酚的植物組織、水果果肉、真菌等的 RNA 提取·無(wú)需使用酚氯仿、β-巰基乙醇等有毒試劑·無(wú)需耗時(shí)的醇類(lèi)沉淀，可以快速提取總 RNA·過(guò)濾柱技術(shù)快速去除組織溶解物中的雜質(zhì) 查看產(chǎn)品
·20-30分鐘內(nèi)即可完成凝膠中DNA的回收·最高可以達(dá)到85%的回收效率·核酸純化柱無(wú)須平衡液處理，最大吸附30 μg DNA·溶液中所含的pH指示劑可直觀(guān)地判斷溶液中DNA與硅膠膜的最適宜結(jié)合條件·特別設(shè)計(jì)的純化柱，可用25 μl微量洗脫體積洗脫DNA·應(yīng)用：DNA測(cè)序、基因芯片分析、DNA連接與轉(zhuǎn)化、限制性酶切、標(biāo)記微注射、PCR、體外轉(zhuǎn)錄 查看產(chǎn)品
粒DNA小量試劑盒-ut-20250120044644.jpg)
·8分鐘內(nèi)即可完成質(zhì)粒DNA的制備，無(wú)須酚氯仿抽提·革命性的創(chuàng)新發(fā)明Buffer N8，2分鐘內(nèi)獲得無(wú)漂浮沉淀的上清液·核酸純化柱無(wú)須平衡液處理即可使用·特殊的添加中和指示劑設(shè)計(jì)，使初次使用者也可以獲得最佳的質(zhì)粒抽提效果 查看產(chǎn)品
粒DNA小量試劑盒-ut-20250120044659.jpg)
·核酸純化柱無(wú)須平衡液處理·特殊的添加中和指示劑設(shè)計(jì)，使初次使用者也可以獲得最佳的質(zhì)粒抽提效果·特別設(shè)計(jì)的Buffer W1 洗滌液，更徹底去除包括限制性?xún)?nèi)切酶在內(nèi)的各種殘留的蛋白，適用于包括野生型宿主菌在內(nèi)的各種大腸桿菌菌株·應(yīng)用：測(cè)序、限制性酶切、連接與轉(zhuǎn)化、體外轉(zhuǎn)錄/翻譯、強(qiáng)壯細(xì)胞轉(zhuǎn)染 查看產(chǎn)品
本試劑盒含有全新高效的gDNase，3min高效去除gDNA，讓基因組DNA在反轉(zhuǎn)前高效去除；高效Quant逆轉(zhuǎn)錄酶，僅需15 min即可合成滿(mǎn)足熒光定量實(shí)驗(yàn)的第一鏈cDNA，反轉(zhuǎn)效率可高達(dá)90%。 查看產(chǎn)品
·1小時(shí)內(nèi)完成植物總DNA的制備·特備適合DNA含量低的植物樣本提取DNA·特別針對(duì)多糖、多酚含量高的植物或真菌樣本設(shè)計(jì) 查看產(chǎn)品
經(jīng)糞便保存液穩(wěn)定的糞便樣本可配套Simgen糞便DNA純化試劑盒（配套糞便保存液使用產(chǎn)品，產(chǎn)品代號(hào)Cat.No.4111050）提取糞便DNA。糞便樣本中的DNA經(jīng)糞便保存液穩(wěn)定后可在室溫（15-25℃）保存6個(gè)月以上而免于降解，2-8℃或更低溫度下可存放2年以上保持DNA不被降解。 查看產(chǎn)品